Large-Scale Production of Extracellular Vesicles from High-Density 3D Cell Cultures in Hollow Fiber Perfusion Bioreactors
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Hollow Fiber CellOur Technology
WHY CHOOSE 3D HOLLOW FIBER OVER T FLASKS / ROLLER BOTTLES
Culture more cells in a smaller space. One large FiberCell cartridge can contain 100-200x more cells than one roller bottle.
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50x > T75 flask
100x > Roller bottle
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HOW FIBERCELL SYSTEMS ENSURE YOUR SUCCESS
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TECHNICAL ADVANTAGES OF FIBERCELL SYSTEMS
01
LESS DISRUPTION, LESS WORK & LESS WASTE
Maintenance is only 15 minutes a day. Passage numbers are irrelevant when you can achieve continuous cell culture production for many months, as splitting of cells is not required.
02
CLEAN, CONCENTRATED & SIMPLIFIED HARVEST
HFBRs allow 10-100X higher concentration of secreted products, and reduced apoptosis and shear on cells. This results in significantly lower host cell protein and DNA contamination, along with reduced membrane fragments.
03
UNIQUE 3-D POROUS SUPPORT
Enables in-vivo like conditions for optimal, high-density cell cultivation and cell co-cultivation. Fiber geometry mimics the mammalian capillary bed.
WE CATER TO A LARGE VARIETY OF APPLICATIONS
Discover the real power of Hollow Fiber Cell Culture. Learn more about our full range of applications.
Thanks for the great product! I am actually more impressed with the Bioreactor now using the CDM-HD than I was using FBS. CDM-HD is cheaper than FBS, no purification required (Protein A or Melon gel), and the yields are about the same.
Dr. Erin Bromage, US Veterinary Immune Reagents Network
Given the preliminary success with CDM HD, I jumped straight into developing a protocol to adenovirally transduce cells and then collect a secreted, recombinant protein of interest in 10% CDM. It worked like a charm. CDM HD serves the purpose, and I am very pleased about that.
Matthew Siviski, Orono, ME
Matthew Siviski, Orono, ME
We have been producing extracellular vesicles in conventional cultures. With the FiberCell Systems’ hollow fiber bioreactors, we can now see pellets in the centrifuge tubes. We have never seen anything like that before.
Jeffrey Franklin, Nashville, TN
Jeffrey Franklin, Nashville, TN
My experience with the FiberCell Systems’ hollow fiber model has opened up a new field of research for me to pursue.
Kacy Baran, Silver Spring, MD
Kacy Baran, Silver Spring, MD
I don’t need to go to the lab on weekends to harvest anymore. I have my work life balance back due to the FiberCell Systems.
Too few cells in relation to volume of media can result in lag phase. In the hollow fiber cartridge lag phase can be defined by the cells not consuming glucose but still show significant viability via the trypan exclusion assay. Always seed the cartridge with the recommended cell density. If this is not possible, adjust the volume of media down to keep the cells in culture from being too dilute.If the cells do go into lag phase you can either:
reduce the volume in the reservoir bottle back down to 125 mL
inoculate more cells
wait a few days – sometimes the trick with hollow fiber cell culture is to know when to NOT do something.
Hybridoma cells growing at high density in the C2011 cartridge using CDM-HD medium
Simply perform your harvest as usual. One of the issues that many researchers have when using our system is that they are afraid to harvest out too many cells. The cartridge can be overgrown if you don’t remove cells. After centrifugation your cell pellet should be between 1-3 mL or more (with the C5011 as much as 5 mL). Simply decant off the supernatant and re-suspend the cell pellet in 2 pellet volumes of fresh medium that has been diluted 10% with sterile distilled water. Leave it overnight at room temperature. The cells will consume the medium and also the hypotonicity will slightly squeeze the cells causing them to release any intracellularly sequestered antibody. This will get you an extra few milligrams every time you harvest.
Various components of serum. The major component, BSA binds many substances non-specifically and can be an overwhelming protein burden in some purification schemes, immunoglobulins can interfere with specific antibody purifications and the “other” can have many undefined effects. None of these characteristics will be reproducible from lot to lot.