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I have been using FiberCell Systems cartridges for the production of recombinant proteins in CHO cells for five years. I routinely produce a half-gram of protein in two months in a volume of less than five liters. This type of production would not be possible using conventional methods. FiberCell Systems hollow fiber bioreactors have made an invaluable contribution to my research.
– Dr. James Arthos, Bethesda, Md.
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246 mg of purified recombinant IgG1 from a CHO (DG44) cell line was harvested from the C2011 20 kD MWCO cartridge. Medium was a serum free, protein free formulation similar to CDM-HD. Each harvest was 19 mL in volume; total harvest volume was 304 mL for an average concentration of over 800 micrograms per day per milliliter. The cartridge consumed 1 liter of medium every two days and the culture was maintained for a total of 35 days. Even though cell viability in the harvest was fairly low the expressed protein was very clean as demonstrated by the gel of the un-purified harvest shown below. Contamination with cellular proteins and DNA was quite low.
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Recombinant immunoglobulin produced by 293t cells in our C2011 cartridge. This gel nicely demonstrates the reduction in contaminating serum proteins after switching to CDM-HD. In this case the researcher harvested every two days rather than every day, so the elimination of serum proteins takes a little longer. Note that the IgG is the dominant protein in the unpurified supernatant after the switch to CDM-HD is complete.
![Hexeramized IgG expressed in CHO cells](https://www.fibercellsystems.com/wp-content/uploads/2023/03/Hexeramized-IgG-expressed-in-CHO-cells.jpg)
Hexeramized IgG expressed in CHO cells. 6 IgG subunits held together with 3 IgA tails. The Fv region has been replaced with CD4 receptor. Top trace is the protein expressed in 2-D flasks, bottom trace is the exact same cells, transferred into the hollow fiber bioreactor. In flask culture protein assembly is not complete, in the HFBR more than 90% of the protein is expressed as a properly folded hexamer.
- Both suspension and adherent cell types
- 100x+ higher concentration
- Easily adapt to SFM
- Can provide improved protein folding